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Myc tag Monoclonal Antibody

Datasheet
  • 货号:
    CSB-MA000041M0m
  • 规格:
    ¥1200
  • 图片:
    • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
      Lane 1: Recombinant Myc-tagged fusion protein at 50ng
      Lane 2: Recombinant Myc-tagged fusion protein at 25ng
      Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
      Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
      Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
      Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 50 kd
      Observed band size: 50 kd

    • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunoprecipitating MYC-tag with transfected HEK293
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
      Lane 3: Transfected HEK293 whole cell lysate (10µg)

  • 其他:

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  • 产品描述:
    Myc tag monoclonal antibody CSB-MA000041M0m was produced in the mouse immunized by using the EQKLISEEDL (Myc) synthetic peptide conjugate to KLH as the immunogen. The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag. This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.
    This Myc tag monoclonal antibody was tested in the ELISA, WB, IF and IP. The non-conjugated IgG1 got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like. It doesn’t have species restricted.
  • 别名:
    bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
  • 宿主:
    Mouse
  • 反应种属:
    N/A
  • 免疫原:
    EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
  • 标记方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal
  • w88优德体育手机app登录:抗体亚型:
    IgG1
  • 纯化方式:
    >95%, Protein G purified
  • 克隆号:
    5F92F9
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, WB, IF, IP
  • 推荐稀释比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:100-1:300
    IP 1:1000-1:1500
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

引用文献

问答及客户评论

 客户评论
  • Western blot review for CD146 Monoclonal Antibody
    Tested application: WB
    Test sample: Cells
    Sample volume: 4μg
    Electrophoresis parameters: 100V
    Primary antibody dilution ratio: 1:500
    Review: Antibody specificity is very good.
  • Western blot review for CD146 Monoclonal Antibody
    Tested application: WB
    Test sample: HEK293 cell lysate
    Sample volume: 20μg
    Electrophoresis parameters: 80V/100V
    Primary antibody dilution ratio: 1:1000
    Review: Myc antibody has good specificity.


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