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His-Tag Monoclonal Antibody

Datasheet
  • 货号:
    CSB-MA000159
  • 规格:
    ¥1,000
  • 图片:
    • IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
      Lane 1: untransfected 293 cell lysate
      Lane 2: transfected 293 cell lysate with His-tag fusion protein
      Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
      Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
      Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
      Lane 6: Recombinant protein (E.coli)

    • IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)

    • 2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

  • 其他:

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  • 产品描述:
    His-Tag monoclonal antibody CSB-MA000159 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The immunogen is a short peptide. It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
    This His-Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. An efficient detection and purification system based on fusion proteins can be established using the His tag. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.
  • 宿主:
    Mouse
  • 反应种属:
    N/A
  • 免疫原:
    Synthetic Peptide
  • 标记方式:
    Non-conjugated
  • w88优德体育手机app登录:抗体亚型:
    IgG
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, WB, IF, IP
  • 推荐稀释比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

引用文献

  • When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell. Siqueira RAGB, et al,Toxicon,2019

    Applications: ELISA
    Antibody dilution factor: 1: 4000
    Review: Murine B16F10 and L929 cell lines (2× 10 5 ) were fixed on plates and then blocked with 2% BSA solution. After that, Rechistatin, Echistatin, LgRec1 or EGFP (1; 3; 6 and 9μM) were added to the plates followed by incubation for 2 h at 37°C. The binding of the recombinant proteins to the cells was assessed using anti-His (Cusabio®) mAb followed by anti-mouse IgG-peroxidase conjugate (Sigma® ). The reaction was measured by spectrophotometer at 492 nm, the higher the absorbance the grater the binding of the toxins to the cells. A) Binding of recombinant toxins on murine B16F10 melanoma cells; B) Binding of recombinant toxins on murine L929 cells. The results were expressed as mean ± SD (n = 3), ***p < 0.001; **p < 0.01 and *p < 0.05 comparing Rechistatin, Echistatin, LgRec1 and EGFP groups com- pared with PBS.
    PMID: 31251993

  • Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus. Huang D, et al,Nature Plants,2018

    Application: Pull-down assay
    Review: Competitive binding assays of CgRuby1 and CgRuby2Short binding to CgbHLH1. The mixture of HIS-CgRuby1 and FLAG CgRuby2Short was added to immobilized GSTCgbHLH1. The precipitates were detected using western blot analysis with anti-HIS, anti-FLAG or anti-GST antibodies. The gradient indicates the increasing amount of FLAG-CgRuby2Short. These experiments were repeated independently twice with similar results.
    PMID: 30374094



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