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E-Tag Monoclonal Antibody

Datasheet
  • 货号:
    CSB-MA000151M0m
  • 规格:
    ¥12,00
  • 图片:
    • Western Blot
      Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Western Blot
      Positive WB detected in: E-tagged fusion protein
      All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
      Lane 3: 293F transfected whole cell lysate (20µg)

       

    • ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
      Antigen at dilution of 1µg/ml
      E-Tag antibody at (0-0.15µg)
      Goat polyclonal to Mouse IgG at 1/50000 dilution

       

    • Western Blot
      Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 30, 68, 55, 67-157kDa
      Observed band size: 30, 68, 55, 67-157kDa

  • 其他:

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  • 别名:
    E-Tag
  • 宿主:
    Mouse
  • 反应种属:
    All
  • 免疫原:
    GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
  • 标记方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal Antibody
  • w88优德体育手机app登录:抗体亚型:
    IgG1
  • 纯化方式:
    >95%, Protein G purified
  • 克隆号:
    11H12B3
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, WB, IP
  • 推荐稀释比:
    Application Recommended Dilution
    ELISA 1:4000-1:8000
    WB 1:1000-1:320000
    IP 1μg-5μg
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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