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Recombinant Human Axin-1(AXIN1) ,partial

  • 货号:
    CSB-YP517675HU
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 货号:
    CSB-EP517675HU
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 货号:
    CSB-EP517675HU-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 货号:
    CSB-BP517675HU
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 货号:
    CSB-MP517675HU
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

优德亚洲平台

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
    AXIN1
  • Uniprot No.:
  • 别名:
    AI316800; AXIN ; Axin 1; Axin-1; axin1; AXIN1_HUMAN; Axis inhibition protein 1; Axis Inhibitor 1 ; Fu; fused; Fused; mouse; homolog of; hAxin; Kb; Ki; kinky; knobbly; MGC132911; MGC52315; PPP1R49; Protein Fused; Protein phosphatase 1 regulatory subunit 49
  • 种属:
    Homo sapiens (Human)
  • 【优德线上娱乐场】蛋白长度:
    Partial
  • 【优德线上娱乐场】蛋白标签:
    The following tags are available.
    N-terminal His-tagged
    Tag-Free
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

靶点详情

  • 功能:
    Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling
  • 基因功能参考文献:
    1. RNF146 exerted oncogenic role through ubiquitination of Axin1 to activate beta-catenin signaling. PMID: 29932918
    2. TRIM65 exerted oncogenic activities via ubiquitylation of Axin1 to activate the beta-catenin signaling pathway. PMID: 28754688
    3. coexpression of APC5 and Axin genes significantly downregulated Wnt signaling in human SW480 CRC cells and inhibited cell growth. PMID: 28731177
    4. data delineate opposing roles for gamma-Pcdh isoforms in regulating Wnt signalling and identify Axin1 as a novel protein interactor of the widely-expressed gamma-Pcdh-C3 isoform PMID: 27530555
    5. Genetic association of polymorphisms in AXIN1 gene with clear cell renal cell carcinoma in a Chinese population. PMID: 29053018
    6. Colorectal cancer cell lines identified VACO6 cells as a carrier of a canonical PTPRK(e1)-RSPO3(e2) fusion; cell line displayed marked in vitro and in vivo sensitivity to WNT blockade by the porcupine inhibitor LGK974. Long-term treatment of VACO6 cells with LGK974 led to the emergence of a resistant population carrying two frameshift deletions of the WNT pathway inhibitor AXIN1, with consequent protein loss. PMID: 28100566
    7. role in the formation of degradasomes and degradation of beta-catenin induced by the TNKSi G007-LK in colorectal cancer cells PMID: 28107521
    8. RUNX1 and AXIN1 proteins are strongly correlated in ER(-) tumors as well. PMID: 28055379
    9. Mutagenesis and binding studies using the bivalent TNKS binding domain of Axin1 demonstrate that only certain ankyrin repeat clusters combinations function together. SAXS analysis is consistent with a dynamic ensemble of TNKS ankyrin repeat conformations modulated by Axin1 interaction. PMID: 27594684
    10. LGALS3 and AXIN1 genes affect tumor sizes and the mucinous component via Wnt/ beta-catenin pathway in the pathogenesis of colorectal cancer PMID: 26894286
    11. The AXIN1 rs1805105 SNP was associated with small tumor size and early tumor stage in hepatocellular carcinoma. PMID: 26968103
    12. AXIN1 is expressed in meningioma along with APC and E-cadherin PMID: 27630299
    13. Data show that poly(ADP-ribose) polymerase (PARP) enzyme Tankyrase (TNKS) inhibition in colon cancer cells decreases beta-catenin signaling at the level of both Axin and APC2. PMID: 27068743
    14. Cancer point mutations in the scaffold protein Axin derail Wnt signaling and promote tumor growth in vivo through a gain-of-function mechanism. PMID: 26974125
    15. The pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells. PMID: 27138857
    16. Data show that 5-hydroxytryptamine receptor (5-HT1DR) played an important role in cell invasion via Axin1/beta-catenin/matrix metalloproteinase 7 (MMP-7) pathway. PMID: 26214021
    17. GSK3 acts through APC motifs R2 and B to regulate APC:Axin interactions, promoting high-throughput of betacatenin to destruction. PMID: 26393419
    18. We have offered primary evidence that the T allele and T/T genotype of rs370681 polymorphisms and C/T genotype of rs1805105 polymorphisms in AXIN1 gene are more frequent in patients with cryptorchidism. PMID: 25802106
    19. Higher expression of AXIN1 protein in IUGR placentae indicates a role of Wnt/beta-catenin signaling pathway in pathology of placental development. PMID: 24846767
    20. This study study showed that HMGA2 was able to elicit EMT and regulate several genes which are closely related to the Wnt/beta-catenin pathway by directly binding to their promoter. PMID: 23135750
    21. Significantly increased atrial septal defect risk was observed to be associated with the A allele of rs12921862 PMID: 25355064
    22. suggest a novel mechanism of Wnt signaling through the dissociation of the beta-catenin destruction complex by Axin1 Thr160 modification PMID: 25735981
    23. In adenomatous polyposis coli, beta-catenin phosphorylation involves Axin and the 20 amino acids of APC or APCL. PMID: 24722208
    24. Which destabilize beta-catenin through stabilizing Axin. PMID: 24419084
    25. Gastric neoplasia of chief cell-predominant type (GN-CCP) as a unique variant of nonaggressive tumor are characterized by nuclear beta-catenin accumulation and mutation of CTNNB1 or AXIN gene, suggesting activation of the Wnt/beta-catenin pathway. PMID: 24011952
    26. The expression levels of CTNNB1, AXIN1 and APC did not correlate with the beta-catenin mutation status, which corroborates previous gene expression micro-array analyses PMID: 23525311
    27. methylation status of the Axin gene may be one important factor to predict radiosensitivity of lung cancer. PMID: 23915259
    28. In invasive breast cancer preserved Axin expression is associated with a more aggressive phenotype and reduced survival. PMID: 23278310
    29. Our study revealed that alterations of AXIN1 were involved in hepatitis B virus-related hepatocellular carcinoma PMID: 23879168
    30. X-radiation and siRNA inhibit expression of HDAC1 and HDAC2, weaken the inhibitory effect of HDAC on Axin, upregulate Axin expression and induce apoptosis of lung cancer cells PMID: 23110995
    31. Abnormal hypermethylation of Axin gene is associated with lung cancer. PMID: 23192643
    32. HectD1 promotes the APC-Axin interaction to negatively regulate Wnt signaling. PMID: 23277359
    33. Despite the absence of AXIN1 gene mutations, the low level of Axin-1 protein in the cytoplasm probably also plays a role in tumorigenesis. PMID: 21769465
    34. Phosphorylation of axin-1 by glycogen synthase kinase(GSK)-3beta accelerates the association of axin-1 with beta-catenin. PMID: 22933777
    35. respiratory epithelial polygonal and cuboidal cells have distinct expression of beta-catenin, Axin, and C-myc PMID: 22614067
    36. Low axin1 is associated with hepatocellular carcinoma. PMID: 22524844
    37. Results demonstrate that beta-catenin is not only phosphorylated inside the APC/Axin1 destruction complex, but also ubiquinated and degraded via the proteasome, all within an intact Axin1 complex. PMID: 22682247
    38. 3 synonymous alterations (p.P39P, p.D260D, p.V383V)were found in patients with ALL and one novel synonymous alteration (p.G67G) in the cohort with AML. A new g.G42525A variation was seen in intron 4-5 in 3 patients with acute leukemia. PMID: 21902576
    39. Wnt/beta-catenin signaling and AXIN1 may regulate the efficacy of inhibitors of BRAF(V600E), suggesting that manipulation of the Wnt/beta-catenin pathway could be combined with BRAF inhibitors to treat melanoma PMID: 22234612
    40. These results reveal an important mode of Myc activation in human breast cancer and a mechanism contributing to Myc deregulation involving unique insight into inactivation of the Axin1 tumor suppressor in breast cancer. PMID: 21808024
    41. RNF146 RING-type ubiquitin E3 ligase as a positive regulator of Wnt signaling that operates with tankyrase to maintain low steady-state levels of Axin proteins PMID: 21799911
    42. Axin I expression was investigated in 42 patients with glioblastoma, the most aggressive form of glial tumors. Immunostaining and image analysis revealed the quantity and localization of the protein. PMID: 21648318
    43. methylation of Axin by PRMT1 may serve as a finely tuned regulation mechanism for Wnt/beta-catenin signaling. PMID: 21242974
    44. axin associates with ubiquitin-specific protease 34 (USP34); results indicate USP34 controls the levels of axin and positively modulate Wnt signaling by acting downstream of beta-catenin stabilization through controlling the nuclear accumulation of axin PMID: 21383061
    45. found that dominant-negative mutants of PML blocked AXIN-induced p53 activation, and that AXIN promotes PML sumoylation, a modification necessary for PML functions PMID: 21057547
    46. these results support a model in which the unfolded nature of these critical scaffolding regions in Axin1 facilitates dynamic interactions with a kinase and its substrate, which in turn act upon each other. PMID: 21087614
    47. Study demonstrate that the DIX domains of Dvl and Axin are expressed noticeably in a multi-cistronic system but not in a mono-cistronic system. PMID: 20846493
    48. Down regulation of axin expression and up regulation of beta-catenin were detected in neuroepithelial brain tumors PMID: 19633924
    49. MAP3K1 functionally interacts with Axin1 in the canonical Wnt signalling pathway PMID: 20128690
    50. Axin downregulates TCF-4 transcription via beta-catenin and independently of p53. PMID: 20122174

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  • 相关疾病:
    Hepatocellular carcinoma (HCC); Caudal duplication anomaly (CADUA)
  • 亚细胞定位:
    Cytoplasm, Nucleus, Membrane, Cell membrane
  • 组织特异性:
    Ubiquitously expressed.
  • 数据库链接:

    HGNC: 903

    OMIM: 114550

    KEGG: hsa:8312

    STRING: 9606.ENSP00000262320

    UniGene: Hs.592082



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