优德亚洲平台

Recombinant Human Novel Coronavirus Spike glycoprotein(S) (V483A), partial

In Stock
  • 货号:
    CSB-MP3324GMY1(M5)
  • 规格:
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    • Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V483A) at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 196.4-272.1 ng/ml.

      Biological Activity Assay

    • ②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V483A) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 is 7.481- 18.76 ng/ml.Biological Activity Assay

  • 其他:

优德亚洲平台

  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V483A) at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 196.4-272.1 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V483A) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 is 7.481- 18.76 ng/ml.
  • Uniprot No.:
  • 别名:
    Spike glycoprotein
  • 种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 【优德线上娱乐场】蛋白长度:
    Partial
  • 来源:
    Mammalian cell
  • 分子量:
    35 kDa
  • 表达区域:
    319-541aa (V483A)
  • 氨基酸序列
    RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGAEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
  • 【优德线上娱乐场】蛋白标签:
    C-terminal 10xHis-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 产品描述:

    The recombinant human novel coronavirus spike glycoprotein (S) (V483A) was generated by the expression of a DNA fragment encoding 319-541AA of human SRAS-CoV-2-S in the mammalian cell. The partial-length protein carries a C-terminal 10xHis-tag. It reached up to 90% in purity determined by SDS-PAGE. It was also verified to be fully biologically active through its interaction with ACE2 and SARS-CoV-2-S Antibody. The endotoxin of this SRAS-CoV-2-S is less than 1.0 EU/μg measured by the LAL method. This recombinant SARS0CoV-2-S protein may be used in the studies of the microbiology research area.

  • Datasheet & COA:
    Please contact us to get it.


千赢国际qy88.vⅰp+qy88.vipmanbetxapp优德亚洲平台beplay3下载